IVF

IVF (IN-VITRO-FERTILIZATION) - AND ICSI (INTRA-CYTOPLASMATIC-SPERM-INJECTION) - TREATMENT

The term In-Vitro derives from Latin, meaning “in-glass”, refers to the fact that the fertilization process occurs outside the human body, in a plastic dish in the embryology laboratory.

In Vitro Fertilization (IVF) technology enhances the chances of conception for couples with fertility problems by bringing thewife’s oocytes (egg) and husband’s sperm together in culture media, to facilitate fertilization and embryo development.

This technique was introduced the late 70’s. Louise Brown was the first baby born using this technique in 1978.In the case of Louise Brown IVF was done because the fallopian tubes of her mother were blocked and she could not conceive naturally. For the IVF-treatment the egg(s) and a specific amount of good motile sperm are mixed in a dish and placed in an incubator. Fertilization has to occur naturally, which means that the sperm have to get inside the egg by themselves.

Meanwhile another technique has been developed to overcome male infertility due to impaired semen quality. In this technique a motile, normal shaped sperm is selected, immobilised and placed inside the egg using a fine needle. This technique is micromanipulation and called intra-cytoplasmic sperm injection (ICSI).

IVF / ICSI – treatments are recommended for

  • Patients with failed artificial insemination trials.
  • Female factor, such as lesions or absence of fallopian tube, endometriosis or ovulation problems if no pregnancy is reached after hormonal stimulation.
  • Male factor infertility, in which sperm count, motility (speed)or morphology (shape) does not allow  normal conception.
  • Unexplained infertility: failure to conceive after several years of attempts without a scientific cause for the infertility.

Procedures required for IVF / ICSI treatment:

Ovarian Stimulation

One or two eggs are produced by the ovaries every month in the natural menstrual cycle. In an IVF cycle the aim is to obtain more eggs,and thus to be able to create more embryos, by the administration of hormones to stimulate egg production by the ovaries

Follicular Puncture (oocyte pickup or egg retrieval)

The development of follicles will be monitored regularly by ultrasound. This will allowfor the best time for the triggering of ovulation by the application of a hormonal injection called human Chorionic Gonadotrophin (hCG). The egg collection (Follicular Puncture) will be scheduled 34-36 hours after this injection. The egg collection procedure is performed using a fine ultrasound-guided needle, underlocal anesthesia or in some cases under general anesthesia.

Oocyte Fertilization and Embryo Culture

The same day of oocyte pick-up procedure, the husband has to provide a semen sample, which is prepared and used for fertilizing the oocyte either by introducing a specific amount of sperm to the eggs in culture media dishes (IVF) or by injecting each oocyte with a single sperm (ICSI). In both ways the oocytes will be cultured overnight in incubators with controlled parameters of temperature and gas concentration to achieve hopefully good quality embryos.

To ensure that there is no mixing up of the sperm and eggs, our lab is working with “RI-Witness” system. Moreover, all the incubators in the lab are continuously monitored externally by the “Xiltrix system” to ensure a safe environment for the embryos.

Embryo Transfer

The embryos are evaluated daily to monitor their progress. To determine the best day for transfer, several factors have to be considered:

  • previous treatment history
  • the development of the embryos (cell number and quality)
  • and also whether any genetic testing will be done on the embryo

The physician and the embryologist will decide when to transfer the embryo(s) to ensure that the patient has the best chance of pregnancy.

The embryo(s) is/are transferred under ultrasound guidance into the uterus by using a thin catheter. It is a simple, and painless procedure and sedation or anesthesia is not required. It is recommended to move immediately after the embryo transfer and not to remain in the bed.

After embryo transfer the patients can contact our qualified experts for any concern, and will be contacted and reminded to do a blood pregnancy test 14 days later.

To seek a consultation with an IVI expert:

Call us at +971 2 666 7049 || Email at info@ivivf.com

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